Mouse arcuate AGRP neurons — fed vs fasting (RNA-seq)
Mus musculus · Arcuate nucleus · RNA-seq (sorted AGRP neurons)
Selected data and study scope
This dataset contains RNA-seq (sorted AGRP neurons) data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Fasted relative to Fed.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate AGRP neurons (FACS), includes 5 Fed samples and 6 Fasted samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The preparation enriches the selected cell population. Interpret expression within that population. Check the source for sorting markers and sample pooling.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse arcuate AGRP neurons — fed vs fasting (RNA-seq); all 1 registered contrasts on default track agrp
Experimental design passed. Mouse arcuate AGRP neurons — fed vs fasting (RNA-seq); all 1 registered contrasts on default track agrp
Author concordance passed. Matched gene effects; fast_vs_fed. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
FACS-purified arcuate AGRP neurons, fed vs fasting — energy-sensing neuron transcriptomics (GSE68177)
Source publication
Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss.
Henry FE, Sugino K, Tozer A, Branco T, Sternson SM
eLife · 2015
Repository study title
Cell type transcriptomics of hypothalamic energy-sensing neuron responses to fasting
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Fasted vs Fed · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Fed n | Fasted n |
|---|---|---|---|
| Arcuate AGRP neurons (FACS) | 14,630 | 5 | 6 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.