Mouse arcuate nucleus — chronic corticosterone
Mus musculus · Arcuate nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Four-week corticosterone relative to Four-week vehicle.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate nucleus (LCM), includes 3 Four-week vehicle samples and 4 Four-week corticosterone samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse arcuate nucleus — chronic corticosterone; all 1 registered contrasts on default track arc
Experimental design passed. Mouse arcuate nucleus — chronic corticosterone; all 1 registered contrasts on default track arc
Author concordance passed. Matched gene effects; corticosterone_4week_vs_vehicle. P values and FDR are contextual. Numerical acceptance: matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Laser-captured arcuate-nucleus RNA-seq after four weeks of oral corticosterone, comparing four treated with three vehicle male mice after the authors' three documented outlier exclusions. The two-day arm is excluded (PMID 31176677; GSE128148)
Source publication
Global transcriptomic analysis of the arcuate nucleus following chronic glucocorticoid treatment.
Wray JR, Davies A, Sefton C, Allen TJ, Adamson A, Chapman P, et al.
Molecular metabolism · 2019
Repository study title
Transcriptomic response of the arcuate nucleus to oral corticosterone treatment
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Four-week corticosterone vs Four-week vehicle · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Four-week vehicle n | Four-week corticosterone n |
|---|---|---|---|
| Arcuate nucleus (LCM) | 11,182 | 3 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.