Mouse postnatal arcuate nucleus — Mettl14 KO (scRNA-seq)
Mus musculus · Arcuate nucleus · scRNA-seq
Selected data and study scope
This dataset contains scRNA-seq data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Mettl14 KO relative to Control.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate nucleus (mouse pseudobulk), includes 4 Control samples and 4 Mettl14 KO samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The source assay profiles individual cells. The track and sample counts shown here describe the analysis used for the comparison. Cell counts do not replace independent biological replicates.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC review needed. Mouse postnatal arcuate nucleus — Mettl14 KO (scRNA-seq); all 1 registered contrasts on default track arcuate
Experimental design review needed. Mouse postnatal arcuate nucleus — Mettl14 KO (scRNA-seq); all 1 registered contrasts on default track arcuate
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
P0 arcuate-median-eminence Drop-seq from four control and four Mettl14 neural-stem-cell knockout mice; sections and 2-3 droplet runs were pooled only within each animal, and 208,711 cells were aggregated to eight mouse pseudobulks (Liu et al., Cell Stem Cell 2025, PMID 40112816; GSE289461)
Source publication
Shen Y, Wong SZH, Ma T, Zhang F, Wang Q, Kawaguchi R, et al.
Cell stem cell · 2025
Repository study title
Single-cell gene expression profile of the postnatal mouse arcuate nucleus in the hypothalamus
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Mettl14 KO vs Control · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Control n | Mettl14 KO n |
|---|---|---|---|
| Arcuate nucleus (mouse pseudobulk) | 17,515 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.