Mouse arcuate neurons — postnatal development
Mus musculus · Arcuate nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Female NeuN-positive PND35 relative to Female NeuN-positive PND12.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate NeuN-positive nuclei, includes 5 Female NeuN-positive PND12 samples and 5 Female NeuN-positive PND35 samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The preparation enriches the selected cell population. Interpret expression within that population. Check the source for sorting markers and sample pooling.
Expression values use DESeq2 variance-stabilized expression. Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse arcuate neurons — postnatal development; all 1 registered contrasts on default track arc_neun
Experimental design passed. Mouse arcuate neurons — postnatal development; all 1 registered contrasts on default track arc_neun
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
RNA-seq of FANS-sorted NeuN-positive arcuate nuclei from five independent four-mouse tissue pools at PND12 and five at PND35, all female. The primary sampling selected one mouse per sex per litter; each pool counts once. Male, NeuN-negative, and WGBS samples are excluded; the deposited DESeq2 variance-stabilized values are analyzed unchanged (PMID 36170368; GSE189945)
Source publication
MacKay H, Gunasekara CJ, Yam KY, Srisai D, Yalamanchili HK, Li Y, et al.
Science advances · 2022
Repository study title
Sex-specific epigenetic development in the mouse hypothalamic arcuate nucleus pinpoints human genomic regions associated with body mass index
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Female NeuN-positive PND35 vs Female NeuN-positive PND12 · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Female NeuN-positive PND12 n | Female NeuN-positive PND35 n |
|---|---|---|---|
| Arcuate NeuN-positive nuclei | 23,480 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.