Mouse hypothalamus — choroid-plexus Ins2 overexpression (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Choroid-plexus Ins2 overexpression relative to Untreated control.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 5 Untreated control samples and 5 Choroid-plexus Ins2 overexpression samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(DESeq2-normalized count + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — choroid-plexus Ins2 overexpression (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design not assessed. Mouse hypothalamus — choroid-plexus Ins2 overexpression (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Wild-type C57BL/6J-mouse hypothalamus two months after choroid-plexus Ins2 overexpression, five control and five overexpression RNA profiles. A library-to-individual-mouse or disjoint-pool declaration remains unresolved; distinct BioSamples alone do not establish it. Choroid-plexus libraries are excluded (PMID 37515772; GSE218188)
Source publication
Mazucanti CH, Kennedy V Jr, Premathilake HU, Doyle ME, Tian J, Liu QR, et al.
Cell reports · 2023
Repository study title
Choroid Plexus-Derived Insulin Inhibits Feeding via Direct Signaling through Arcuate Nucleus
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Choroid-plexus Ins2 overexpression vs Untreated control · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Untreated control n | Choroid-plexus Ins2 overexpression n |
|---|---|---|---|
| Whole hypothalamus | 20,752 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.