Mouse hypothalamus — lactational heat stress
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Heat stress, 36 °C relative to Control, 25 °C.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 8 Control, 25 °C samples and 8 Heat stress, 36 °C samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(FPKM + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — lactational heat stress; all 1 registered contrasts on default track hypothalamus
Experimental design passed. Mouse hypothalamus — lactational heat stress; all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole-hypothalamus RNA-seq from eight lactating-day-13 mice housed at 36 °C versus eight at 25 °C. Pituitary and mammary-gland libraries are excluded; official per-sample FPKM values are analyzed as log2(FPKM+1) without rounding (PMID 31480864; GSE128660)
Source publication
Han J, Shao J, Chen Q, Sun H, Guan L, Li Y, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019
Repository study title
Transcriptome of heat-stressed lactating mice in hypothalamus, pituitary and mammary gland
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Heat stress, 36 °C vs Control, 25 °C · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Control, 25 °C n | Heat stress, 36 °C n |
|---|---|---|---|
| Whole hypothalamus | 33,714 | 8 | 8 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.