Mouse hypothalamic oxytocin neurons — CCK-8s (RiboTag)
Mus musculus · Whole hypothalamus · RiboTag translatome
Selected data and study scope
This dataset contains RiboTag translatome data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in CCK-8s relative to Vehicle.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Oxytocin neurons (RiboTag), includes 4 Vehicle samples and 3 CCK-8s samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
This preparation profiles ribosome-associated RNA from the targeted cells. Treat the result as a translatome measurement. It does not represent total RNA from every cell in the anatomical region.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamic oxytocin neurons — CCK-8s (RiboTag); all 1 registered contrasts on default track oxytocin
Experimental design passed. Mouse hypothalamic oxytocin neurons — CCK-8s (RiboTag); all 1 registered contrasts on default track oxytocin
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Hypothalamic oxytocin-neuron RiboTag translatome on standard chow, vehicle vs CCK-8s (20 ug/kg i.p., 2 h) — the gut-to-brain satiation arm. The chow-vs-HFHS arm of the same series is deliberately NOT used: oxytocin-neuron IP purity failed in half the HFHS libraries and every IGF-panel gene tracks that purity, which would have produced an artefactual diet effect. Libraries are pools of 2-3 hypothalami (Gruber et al., Cell Rep 2023, PMID 37864798; GSE242245)
Source publication
Gruber T, Lechner F, Murat C, Contreras RE, Sanchez-Quant E, Miok V, et al.
Cell reports · 2023
Repository study title
Effect of CCK-8s injection (20 ug/kg BW i.p.) on mRNA translational activity in hypothalamic oxytocin neurons of mice fed standard chow or high-fat/high-sugar diet (RiboTag;TRAP)
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
CCK-8s vs Vehicle · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Vehicle n | CCK-8s n |
|---|---|---|---|
| Oxytocin neurons (RiboTag) | 17,013 | 4 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.