Mouse hypothalamus — female pubertal transition
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Female PND37 relative to Female PND22.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 5 Female PND22 samples and 5 Female PND37 samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — female pubertal transition; all 1 registered contrasts on default track hypothalamus
Experimental design passed. Mouse hypothalamus — female pubertal transition; all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole-hypothalamus 3-prime UTR RNA-seq comparing five female mice at PND22 with five at PND37. Male and other developmental-stage libraries are excluded from the focused female pubertal-transition contrast (PMID 36221127; E-MTAB-12340 / PRJEB57087)
Related methods publication
Postnatal developmental trajectory of sex-biased gene expression in the mouse pituitary gland.
Hou H, Chan C, Yuki KE, Sokolowski D, Roy A, Qu R, et al.
Biology of sex differences · 2022
The hypothalamus repository record references this pituitary paper for the shared mouse cohort, protocols and analysis methods. It is a related methods publication, not a publication of the hypothalamus dataset itself; the paper's deposited datasets are E-MTAB-9459 and E-MTAB-9460.
Repository study title
3'-UTR-seq of Mus musculus female and male hypothalamus across the pubertal transition
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Female PND37 vs Female PND22 · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Female PND22 n | Female PND37 n |
|---|---|---|---|
| Whole hypothalamus | 10,863 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.