Mouse medial preoptic PNOC neurons — BacTRAP enrichment
Mus musculus · Preoptic area · BacTRAP translatome
Selected data and study scope
This dataset contains BacTRAP translatome data from Mus musculus, sampled from preoptic area. The default comparison examines gene expression in PNOC-neuron ribosome IP relative to Matched total input.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Medial preoptic PNOC neurons (BacTRAP), includes 3 Matched total input samples and 3 PNOC-neuron ribosome IP samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
This preparation profiles ribosome-associated RNA from the targeted cells. Treat the result as a translatome measurement. It does not represent total RNA from every cell in the anatomical region.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse medial preoptic PNOC neurons — BacTRAP enrichment; all 1 registered contrasts on default track mpoa
Experimental design review needed. Mouse medial preoptic PNOC neurons — BacTRAP enrichment; all 1 registered contrasts on default track mpoa
Author concordance passed. Matched gene effects; pnoc_ip_vs_input. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Medial-preoptic-area BacTRAP from 17-18-week-old female NuTRAP::Pnoc-Cre mice: three independent pools of three animals, with matched total input and PNOC-neuron ribosome-IP libraries modeled as pairs. This is IP enrichment, not a treatment response (GSE306829)
Repository study title
Medial preoptic PNOC neurons constrain brown adipose tissue thermogenesis and energy expenditure
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
PNOC-neuron ribosome IP vs Matched total input · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Matched total input n | PNOC-neuron ribosome IP n |
|---|---|---|---|
| Medial preoptic PNOC neurons (BacTRAP) | 19,802 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.