Mouse VMH — thyroid-hormone-receptor beta knockout
Mus musculus · Ventromedial hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from ventromedial hypothalamus. The default comparison examines gene expression in AAV-Cre Thrb knockout relative to AAV-GFP control.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Ventromedial hypothalamus (VMH), includes 3 AAV-GFP control samples and 4 AAV-Cre Thrb knockout samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2 counts per million (log2-CPM). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse VMH — thyroid-hormone-receptor beta knockout; all 1 registered contrasts on default track vmh
Experimental design passed. Mouse VMH — thyroid-hormone-receptor beta knockout; all 1 registered contrasts on default track vmh
Author concordance passed. Selected author genes only; vmh_thrb_knockout_vs_control. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Ventromedial-hypothalamus RNA-seq from three Thrb-flox mice injected with AAV-GFP and four injected with AAV-Cre, with unique GSM, BioSample, and SRA experiment identifiers. The official TMM-normalized log2-CPM matrix is analyzed unchanged by limma (PMID 28614708; GSE98690)
Source publication
Hameed S, Patterson M, Dhillo WS, Rahman SA, Ma Y, Holton C, et al.
Cell reports · 2017
Repository study title
Thyroid hormone receptor beta in the ventromedial hypothalamus is essential for the physiological regulation of food intake and body weight
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
AAV-Cre Thrb knockout vs AAV-GFP control · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | AAV-GFP control n | AAV-Cre Thrb knockout n |
|---|---|---|---|
| Ventromedial hypothalamus (VMH) | 11,865 | 3 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.