Rat hypothalamus — peri-adolescent high-fructose diet (bulk RNA-seq)
Rattus norvegicus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Rattus norvegicus, sampled from whole hypothalamus. The default comparison examines gene expression in High-fructose diet (55%) relative to Standard chow (0.30% fructose).
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 7 Standard chow (0.30% fructose) samples and 6 High-fructose diet (55%) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(FPKM + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Rat hypothalamus — peri-adolescent high-fructose diet (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design review needed. Rat hypothalamus — peri-adolescent high-fructose diet (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
PND80 male-Wistar-rat whole hypothalamus after standard chow vs 55% fructose diet from PND25, seven chow and six fructose RNA profiles. Rats were pair-housed during diet exposure and selected cage memberships are not deposited. Sequenced animals were fasted, non-stressed and saline-control-injected (PMID 26356038; GSE56238)
Source publication
Harrell CS, Burgado J, Kelly SD, Johnson ZP, Neigh GN
Psychoneuroendocrinology · 2015
Repository study title
Peri-adolescent high-fructose diet effects on hypothalamic transcriptome
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
High-fructose diet (55%) vs Standard chow (0.30% fructose) · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Standard chow (0.30% fructose) n | High-fructose diet (55%) n |
|---|---|---|---|
| Whole hypothalamus | 14,036 | 7 | 6 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.