Chicken hypothalamus — L-citrulline diet

Gallus gallus · Whole hypothalamus · bulk RNA-seq

GSE202003

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Selected data and study scope

This dataset contains bulk RNA-seq data from Gallus gallus, sampled from whole hypothalamus. The default comparison examines gene expression in 1% L-citrulline at 24 °C relative to Basal diet at 24 °C.

The explorer includes 1 track and 1 comparison for this dataset.

The default track, Whole hypothalamus, includes 6 Basal diet at 24 °C samples and 6 1% L-citrulline at 24 °C samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.

Interpretation and limitations

The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.

Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.

Evidence and verification

Pipeline QC passed. Chicken hypothalamus — L-citrulline diet; all 1 registered contrasts on default track hypothalamus

Experimental design passed. Chicken hypothalamus — L-citrulline diet; all 1 registered contrasts on default track hypothalamus

Author concordance passed. Matched gene effects; lcitrulline_vs_basal_thermoneutral. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.

Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.

Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.

Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.

Source study

Broiler whole-hypothalamus RNA-seq comparing six birds fed 1% L-citrulline with six basal-diet birds at 24 °C. The 35 °C arms and workbook FPKM columns are excluded; only author-supplied raw-count columns enter edgeR (PMID 37844531; GSE202003)

Source publication

Transcriptomics analysis unveils key potential genes associated with brain development and feeding behavior in the hypothalamus of L-citrulline-fed broiler chickens.

Uyanga VA, Bello SF, Qian X, Chao N, Li H, Zhao J, et al.

Poultry science · 2023

Repository study title

Transcriptomics of hypothalamic responses of broiler chickens fed L-citrulline supplemented diets under different ambient temperatures

The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.

How to cite the dataset, publication and HypoExplorer

Comparisons in HypoExplorer

1% L-citrulline at 24 °C vs Basal diet at 24 °C · default

Baseline: Basal diet at 24 °C → Case: 1% L-citrulline at 24 °C

Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.

Available tracks

Detected features and sample counts for 1% L-citrulline at 24 °C vs Basal diet at 24 °C. Counts are specific to each track.
Track	Features	Basal diet at 24 °C n	1% L-citrulline at 24 °C n
Whole hypothalamus	11,184	6	6

Expression and analysis results

Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.

HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.