Mouse AgRP neurons — 24-hour fasting at ZT14 (RiboTag RNA-seq)
Mus musculus · Arcuate nucleus · RiboTag translatome
Selected data and study scope
This dataset contains RiboTag translatome data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Fasted (24 h) relative to Ad libitum fed.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate AgRP neurons (RiboTag), includes 4 Ad libitum fed samples and 5 Fasted (24 h) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
This preparation profiles ribosome-associated RNA from the targeted cells. Treat the result as a translatome measurement. It does not represent total RNA from every cell in the anatomical region.
Expression values use log2(DESeq2-normalized count + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse AgRP neurons — 24-hour fasting at ZT14 (RiboTag RNA-seq); all 1 registered contrasts on default track agrp
Experimental design review needed. Mouse AgRP neurons — 24-hour fasting at ZT14 (RiboTag RNA-seq); all 1 registered contrasts on default track agrp
Author concordance passed. Selected author genes only; fast24h_vs_fed. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Ribosome-bound RNA from AGRP-Cre;RiboTag arcuate neurons collected at ZT14, four ad-libitum-fed versus five 24-hour-fasted pooled RiboTag profiles; independence of the pooled preparations remains under review. Time, genotype, and affinity fraction are fixed (GSE124693)
Source publication
Transcriptional Basis for Rhythmic Control of Hunger and Metabolism within the AgRP Neuron.
Cedernaes J, Huang W, Ramsey KM, Waldeck N, Cheng L, Marcheva B, et al.
Cell metabolism · 2019
Repository study title
Coordination of hunger and sleep through circadian regulation of transcription in energy-sensing neurons
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Fasted (24 h) vs Ad libitum fed · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Ad libitum fed n | Fasted (24 h) n |
|---|---|---|---|
| Arcuate AgRP neurons (RiboTag) | 18,611 | 4 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.