Mouse arcuate nucleus — 24-hour food deprivation (bulk RNA-seq)
Mus musculus · Arcuate nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Food deprived (24 h) relative to Ad libitum fed.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate nucleus (laser capture), includes 5 Ad libitum fed samples and 5 Food deprived (24 h) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(RPKM + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse arcuate nucleus — 24-hour food deprivation (bulk RNA-seq); all 1 registered contrasts on default track arcuate
Experimental design passed. Mouse arcuate nucleus — 24-hour food deprivation (bulk RNA-seq); all 1 registered contrasts on default track arcuate
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Laser-capture-microdissected arcuate nucleus from male C57BL/6 mice, ad-libitum fed vs 24-hour food deprived, n=5/5 independent animals. Other hypothalamic nuclei in the series are excluded (GSE96627)
Repository study title
Transcriptomic response to 24-hour food deprivation in four hypothalamic nuclei
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Food deprived (24 h) vs Ad libitum fed · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Ad libitum fed n | Food deprived (24 h) n |
|---|---|---|---|
| Arcuate nucleus (laser capture) | 29,865 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.