Mouse hypothalamus — Akkermansia intervention (scRNA-seq)
Mus musculus · Whole hypothalamus · scRNA-seq
Selected data and study scope
This dataset contains scRNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Fructose + A. muciniphila relative to Fructose control.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus (Drop-seq pseudobulk), includes 3 Fructose control samples and 3 Fructose + A. muciniphila samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The source assay profiles individual cells. The track and sample counts shown here describe the analysis used for the comparison. Cell counts do not replace independent biological replicates.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — Akkermansia intervention (scRNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design not assessed. Mouse hypothalamus — Akkermansia intervention (scRNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole-hypothalamus Drop-seq from 10-week-old DBA/2J mice fed fructose, three control and three Akkermansia muciniphila libraries; 47,492 cells were aggregated per source library, and library-to-animal or pool identity remains unestablished (Ha et al., bioRxiv 2022, GSE212491)
Source publication · preprint
Sung Min Ha, In-Sook Ahn, Thomas Kowal-safron, Justin Yoon, Christine A. Olson, Graciel Diamante, et al.
2022
Repository study title
Multi-omics analysis reveals gut-brain interactions mediating the anti-obesity effect of Akkermansia muciniphila
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Fructose + A. muciniphila vs Fructose control · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Fructose control n | Fructose + A. muciniphila n |
|---|---|---|---|
| Whole hypothalamus (Drop-seq pseudobulk) | 15,564 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.