Aged mouse hypothalamus — caloric restriction (spatial RNA-seq)
Mus musculus · Whole hypothalamus · spatial RNA-seq
Selected data and study scope
This dataset contains spatial RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Caloric restriction relative to Ad libitum.
The data compare caloric restriction with ad libitum feeding in 24-month-old male mice. The analysis aggregates hypothalamic spatial profiles from two sections for each independent animal.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Hypothalamus (spatial animal pseudobulk), includes 3 Ad libitum samples and 3 Caloric restriction samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The analysis units are animals, rather than individual spatial profiles. This comparison concerns dietary restriction in aged males.
The source assay measures spatial profiles. Before interpreting sample counts or differential expression, check how profiles and sections are grouped into analysis samples.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Aged mouse hypothalamus — caloric restriction (spatial RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design passed. Aged mouse hypothalamus — caloric restriction (spatial RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance passed. Selected author genes only; cr_vs_al. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Male 24-month-old mouse brain IRISeq, hypothalamic spots from two sections aggregated to independent animals, ad libitum n=3 vs caloric restriction n=3 (Zhang et al., Cell Rep 2025, PMID 40833855; GSE273038)
Source publication
Spatiotemporal profiling reveals the impact of caloric restriction in the aging mammalian brain.
Zhang Z, Epstein A, Schaefer C, Abdulraouf A, Jiang W, Zhou W, et al.
Cell reports · 2025
Repository study title
Spatiotemporal profiling reveals the impact of caloric restriction in the aging mammalian brain
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Caloric restriction vs Ad libitum · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Ad libitum n | Caloric restriction n |
|---|---|---|---|
| Hypothalamus (spatial animal pseudobulk) | 13,941 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.