Piglet hypothalamus — formula composition
Sus scrofa · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Sus scrofa, sampled from whole hypothalamus. The default comparison examines gene expression in Alpha-lactalbumin-enriched formula relative to Whey-protein-isolate formula.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 12 Whey-protein-isolate formula samples and 11 Alpha-lactalbumin-enriched formula samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC review needed. Piglet hypothalamus — formula composition; all 1 registered contrasts on default track hypothalamus
Experimental design review needed. Piglet hypothalamus — formula composition; all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
P16 piglet hypothalamus RNA-seq comparing 11 alpha-lactalbumin-enriched-formula animals with 12 whey-protein-isolate-formula controls, adjusted for sex. Sow-fed animals are excluded to hold the rearing environment constant (PMID 41102179; GSE281726)
Source publication
Functional gut variability drives metabolic and transcriptional features in neonatal piglets.
He X, Shoff S, Lee H, Hartvigsen ML, Kvistgaard AS, Slupsky CM
NPJ science of food · 2025
Repository study title
Effect of Early Diet on Gene Expression in Gastrointestinal Tract, Liver and Brain Regions of Neonatal Piglets
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Alpha-lactalbumin-enriched formula vs Whey-protein-isolate formula · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Whey-protein-isolate formula n | Alpha-lactalbumin-enriched formula n |
|---|---|---|---|
| Whole hypothalamus | 9,460 | 12 | 11 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.