Rat fetal hypothalamus — maternal ozone exposure
Rattus norvegicus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Rattus norvegicus, sampled from whole hypothalamus. The default comparison examines gene expression in Maternal 0.8-ppm ozone relative to Maternal filtered air.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole fetal hypothalamus, includes 6 Maternal filtered air samples and 6 Maternal 0.8-ppm ozone samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(CPM + 0.0001). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Rat fetal hypothalamus — maternal ozone exposure; all 1 registered contrasts on default track fetal_hypothalamus
Experimental design review needed. Rat fetal hypothalamus — maternal ozone exposure; all 1 registered contrasts on default track fetal_hypothalamus
Author concordance review needed. Selected author genes only; female_fetal_ozone_vs_air. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Female GD21 fetal whole-hypothalamus RNA-seq comparing six maternal 0.8-ppm ozone exposures with six filtered-air controls. The author-provided log2(CPM+0.0001) values are analyzed unchanged; male fetuses are excluded (PMID 33031959; GSE156859)
Source publication
Miller CN, Dye JA, Henriquez AR, Stewart EJ, Lavrich KS, Carswell GK, et al.
Molecular metabolism · 2020
Repository study title
Sexually dimorphic ozone-induced changes in fetal rat hypothalamus
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Maternal 0.8-ppm ozone vs Maternal filtered air · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Maternal filtered air n | Maternal 0.8-ppm ozone n |
|---|---|---|---|
| Whole fetal hypothalamus | 11,083 | 6 | 6 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.