Rat supraoptic nucleus — 72-hour water deprivation (bulk RNA-seq)
Rattus norvegicus · Supraoptic nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Rattus norvegicus, sampled from supraoptic nucleus. The default comparison examines gene expression in Water deprived (72 h) relative to Euhydrated.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Supraoptic nucleus (SON), includes 5 Euhydrated samples and 5 Water deprived (72 h) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC review needed. Rat supraoptic nucleus — 72-hour water deprivation (bulk RNA-seq); all 1 registered contrasts on default track son
Experimental design review needed. Rat supraoptic nucleus — 72-hour water deprivation (bulk RNA-seq); all 1 registered contrasts on default track son
Author concordance passed. Matched gene effects; dehydrated_vs_euhydrated. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Male Wistar-Han rat supraoptic nucleus, euhydrated vs 72-hour water deprivation, five rat profiles per arm; cages were randomized to treatment, and sample-to-cage allocation remains unresolved (PMID 34297454; GSE175461)
Source publication
Pauža AG, Mecawi AS, Paterson A, Hindmarch CCT, Greenwood M, Murphy D, et al.
Journal of neuroendocrinology · 2021
Repository study title
Transcriptome of hypothalamic supraoptic nucleus in response to 72h water deprivation in Wistar Han rats
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Water deprived (72 h) vs Euhydrated · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Euhydrated n | Water deprived (72 h) n |
|---|---|---|---|
| Supraoptic nucleus (SON) | 13,414 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.