Rat hypothalamus — acute ozone exposure (bulk RNA-seq)
Rattus norvegicus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Rattus norvegicus, sampled from whole hypothalamus. The default comparison examines gene expression in Ozone 0.8 ppm (4 h) relative to Filtered air (4 h).
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 4 Filtered air (4 h) samples and 4 Ozone 0.8 ppm (4 h) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(DESeq2-normalized count + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Rat hypothalamus — acute ozone exposure (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design passed. Rat hypothalamus — acute ozone exposure (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole hypothalamus from 13-week-old male Wistar-Kyoto rats after sham surgery, filtered air vs 0.8 ppm ozone for four hours, n=4/4 independent animals. Adrenalectomized and brainstem arms are excluded (PMID 31397875; GSE133293)
Source publication
Ozone-Induced Dysregulation of Neuroendocrine Axes Requires Adrenal-Derived Stress Hormones.
Henriquez AR, House JS, Snow SJ, Miller CN, Schladweiler MC, Fisher A, et al.
Toxicological sciences : an official journal of the Society of Toxicology · 2019
Repository study title
Ozone exposure differentially changed global gene expression at brainstem and hypothalamus in healthy (SHAM) and adrenalectomized rats
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Ozone 0.8 ppm (4 h) vs Filtered air (4 h) · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Filtered air (4 h) n | Ozone 0.8 ppm (4 h) n |
|---|---|---|---|
| Whole hypothalamus | 20,845 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.